News from the lab

more rhomboid inhibitor papers...

08 02 2018

in collaboration with Steven Verhelst and Sascha Weggen

Goel, P., Jumpertz, T., Ticha, A., Ogorek, I., Mikles, D. C., Hubalek, M., Pietrzik, C. U., Strisovsky, K., Schmidt, B., and Weggen, S. (2018)
Discovery and validation of 2-styryl substituted benzoxazin-4-ones as a novel scaffold for rhomboid protease inhibitors.
Bioorganic and Medicinal Chemistry Letters, in press

Yang, J., Barniol-Xicota, M., Nguyen, M. T. N., Ticha, A., Strisovsky, K., and Verhelst, S. H. L. (2018)
Benzoxazin-4-ones as novel, easily accessible inhibitors for rhomboid proteases.
Bioorganic and Medicinal Chemistry Letters, in press

new paper from the group

15 12 2017

on rhomboid inhibitors in Biochemistry

A collaborative paper with Sascha Weggen’s group in Duesseldorf on the discovery of a new scaffold based on modified saccharin that potently inhibits rhomboid proteases “Discovery and Biological Evaluation of Potent and Selective N-Methylene Saccharin-Derived Inhibitors for Rhomboid Intramembrane Proteases” just coming out in Biochemistry.

Maybe they taste sweet? We haven’t tried… ;-)

new paper from the group

15 12 2017

on rhomboid inhibitors in Cell Chemical Biology

Anezka and Stanco’s paper on the discovery of new potent and pharmacologically compliant inhibitors of rhomboid proteases based on peptidyl ketoamides has been published in Cell Chemical Biology.

new paper from the group

05 08 2017

on RHBDL2 in Scientific Reports

http://rdcu.be/uKwN

Congratulations to Nicholas!

David leaves the group

30 06 2017

David Mikles leaves the group to go back to the US at the Moffitt Cancer Center in Tampa, Florida! Good luck.

Květa and Šárka's BSc viva!

06 06 2017

Květa and Šárka have defended their bachelor (BSc) theses with flying colours! What a joy to work with you, ladies!

Aymeric leaves the group

26 05 2017

Aymeric decided to move to France and leaves the group. You will be missed!

new papers from the group

16 01 2017

in Methods in Enzymology

Strisovsky, K. (2017) Mechanism and inhibition of rhomboid proteases. Methods Enzymol. 584, 279-293.

Arutyunova, E., Panigrahi, R., Strisovsky, K., and Lemieux, M. J. (2016) Production of recombinant rhomboid proteases. Methods Enzymol. 584, 255-278.

Arutyunova, E., Strisovsky, K., and Lemieux, M. J. (2016) Activity assays for rhomboid proteases. Methods Enzymol. 584, 395-437.

new paper from the group

16 01 2017

on rhomboid substrates in J Biol Chem

Ticha A, Stanchev S, Skerle J, Began J, Ingr M, Svehlova K, Polovinkin L, Ruzicka M, Bednarova L, Hadravova R, Polachova E, Rampirova P, Brezinova J, Kasicka V, Majer P, Strisovsky K (2017) Sensitive versatile fluorogenic transmembrane peptide substrates for rhomboid intramembrane proteases. J Biol Chem: doi: 10.1074/jbc.M1116.762849.

Here we introduce our new substrates and activity assays for rhomboid proteases. Congratulations to Anezka Ticha and Stancho Stanchev!

Blanka joins the group

20 11 2016

Dr. Blanka Collis is awarded the IOCB postdoctoral fellowship and joins the group as a postdoctoral research fellow. Blanka has done her undergaduate studies at Cambridge University (Pembroke College), her PhD at the University of Sheffield and her first postdoctoral training at Oxford University, UK. Welcome, Blanka! We are looking forward to working with you.

Anicka leaves for Cambridge

01 10 2016

Anicka Suchánková leaves the group to commence her studies at Cambridge University, Queens College. We are sad to let you go, but feel proud about you and wish you all the best!

Great achievement!

new paper from the group

29 08 2016

review on rhomboid inhibitors

In a special issue of Seminars in Cell and Developmental Biology on rhomboid superfamily proteins, Kvido reviews the current status and projects the future of rhomboid protease inhibitors.

Květa and Jolana join the lab

20 06 2016

Our new bachelor students Květa Trávníčková and Jolana Kučerová from Charles University have joined the group. Welcome! We are very happy to have you and hope that you will enjoy working with us and learn a lot!

new paper from the group

06 06 2016

on RHBDL4 and TGFa

In collaboration with the Lemberg, Freeman and Adrain labs in Heidelberg, Oxford and Lisbon,respectively, we have discovered that a human rhomboid protease regulates ER export and secretion of membrane-anchored TGFa in exosome-like vesicles.

Our findings provide a different molecular explanation of the recent observations by Song et al. who find that the same rhomboid protease (RHBDL4) promotes colorectal cancer progression by the autocrine activation of EGFR signalling.

Edita joins the group

15 04 2016

Mgr. Edita Poláchová joins the group as a research assistant. Welcome, Edita! :)

Kumar joins the lab

01 03 2016

as a visiting student

Kumar Tekwani Movellan from the group of Dr. Rasmus Linser joins our lab for a few months to learn how to make recombinant rhomboid proteases by all means and in all forms.! :) Happy to have you here, Kumar!

new paper from the group

04 01 2016

on why cells need intramembrane proteases

Did you ever wonder why cells have intramembrane proteases? Read this new viewpoint article by Kvido to find out.

postdoc fellowship for Aymeric

27 10 2015

Aymeric has been awarded the IOCB Postdoctoral Fellowship. Congratulations!

Šárka and Anna join the lab

10 10 2015

Our new bachelor students Šárka Boháčová and Anna Deylová from Charles University have joined the group. Welcome! We are very happy to have you and hope that you will enjoy working with us and learn a lot.

Prague Membrane Discussions

29 05 2015

2015 part II announcement

Prague Membrane Discussions 2015/II

Venue: ÚOCHB AV ČR, Flemingovo n. 2, Praha 6 ; Seminar room B4.29 (in the new building B)
Date: Thursday June 18th 2015, 10am-2pm

For those who know: second Discussion in the 2015 series, this time organised by Kvido Strisovsky from IOCB and featuring new IOCB groups working on lipid membrane related stuff.

For the newcomers: Prague Membrane Discussions is an informal interest group and discussion forum for scientists interested in lipid membrane related phenomena in some way. The aim is to bring together people from Prague (and around) to foster idea exchange, collaborations etc. Topics covered include model and cell membranes, membrane-associated molecules and processes and various techniques for lipid membrane research. The presenters and audience range from theoretical and (bio)physical chemists to structural and molecular cell biologists. Currently organised by the Cebecauer, Malínský and Stříšovský labs, we gather 3 times per year for short meetings of 3-5 talks and open and lively discussions peppered with tough and rigorous questions. But we are open to considering alternative formats: poster and video presentations, surveys, man-on-man discussions, hands-on practical courses or some other forms of scientific interactions in the future! :)

Preliminary programme

  • special guest Zdeněk Hostomský (IOCB Director): Welcome address
  • Kvido Stříšovský: Introduction
  • Pavel Jungwirth: Cell penetrating peptides (computational)
  • Volodymyr Shvadchak: Interaction of α-synuclein with membranes studied using environment-sensitive fluorescent dyes (expt)

  • Coffee break & roof-garden chill-out excursion (approx. 11:15 – 12:10)
  • Kvido Stříšovský: Intramembrane proteolysis – protein recognition and catalysis within the lipid bilayer (expt)
  • Evžen Bouřa: PI4 kinases – structures and mechanism (expt)
  • Josef Lazar: Uses of polarization microscopy in studies of membrane protein structure and function, and membrane organization (expt)

For all the presentations except for the shorter introductory one we are looking at about 20 min talk + 10 min discussion, which will make it about three hours of talks + a coffee/tea break in between.

Looking forward to seeing you there!

Kvido

Petra joins the group

07 05 2015

Mgr. Petra Rampírová joins the lab as a research assistant. Welcome, Petra!

Aymeric has joined the group

04 05 2015

Dr. Aymeric Morlé has just received his PhD in Molecular and Cellular Biology from the University of Burgundy, France. Having worked in the lab of Carmen Garrido and Olivier Micheau, he now joins our lab as a postdoctoral fellow. Welcome, Aymeric!

postdoc fellowship for David

17 10 2014

David has won the IOCB postdoctoral fellowship. Congratulations!

Jitka joins the group

06 10 2014

Dr. Jitka Novotná joins the group as an investigator scientist. She has done PhD in microbiology and is evolving towards structural biology. Welcome, Jitka!

David joins the group

20 09 2014

Dr. David C. Mikles has recently completed his PhD at the University of Florida, USA, and joins our lab as a postdoc. Welcome, David!

Anička joins the group

10 09 2014

Anna Suchánková is joining us as a research student. She has a keen interest in chemistry, mathematics and volleyball. Wonderful!

Sebastian leaving the lab

15 09 2014

...for a postdoc in Oxford, UK.

Sebastian Zoll is leaving at the end of September to start a second postdoc with Matthew Higgins at the Department of Biochemistry of Oxford University, UK.

Sebastian, thank you very much for all the excellent work you’ve done in the lab! Getting an EMBO Journal paper in less than 2 years starting from scratch, that is just impressive! Continue like this!

All the best.

Lucie has left the lab

15 09 2014

...to do a PhD in Grenoble, FR.

Lucie Peclinovská has left our lab to undertake a PhD in Grenoble in the group of Eva Pebay-Peroula. Membrane protein crystallography adventure ahead! The seed has been sown and germinates! :)

Lucka, thank you very much for all your excellent work. It was very enjoyable to have you in the lab! Completing your masters degree with co-authorship on Nucleic Acids Research and EMBO Journal papers – not bad!

All the best!

Hiring!

13 08 2014

...position filled.

The postdoc position has been just filled. Thanks to all applicants.

Do you know?...

30 07 2014

...where some of the best currently used drugs against HIV and hepatitis B virus originate from? Guess and check out this

Another summer trainee!

29 07 2014

Marie Komrsová from the University of Glasgow has joined us for a short summer placement (whilst being on summer holidays back home :).

Paper highlight

16 07 2014

Sharpening rhomboid specificity by dimerisation and allostery

A new concept proposed in intramembrane proteolysis – regulation of rhomboid activity by dimerisation and allosteric activation. From the lab of Joanne Lemieux, just coming out in The EMBO Journal.

My highlight and commentary article to this paper here.

Also see my Tweet:


We have a summer student!

08 07 2014

an IAESTE trainee

Camilla Linhart from Vienna has spent 6 weeks with us as an IAESTE trainee. She deepened her expertise in DNA cloning, mutagenesis, recombinant protein expression in E.coli, purification of recombinant membrane proteins and enzymatic assays. Almost everything worked! Well done, lots accomplished in a 6-weeks internship! And besides that she has also managed to see the beautiful summery Prague, travel around the country and enjoy sunshine and barbecue with us. Very happy to have had you with us, Camilla! What are you doing next summer?... :)

We have a visitor scientist

11 06 2014

in the lab…

Eliane Kuettler (second from the left) from Steven Verhelst’s lab in Munich has come to our lab for two weeks to finish some experiments for her thesis. Most experiments worked and we really enjoyed having Eliane with us!

Lucie has won a PhD fellowship!

06 06 2014

to go to Grenoble, France

Lucie has decided for a PhD abroad and has won a prestigious fellowship to undertake PhD studies with Eva Pebay-Peyroula at the Institute of Structural Biology in Grenoble, France. Lucie is in love with France and got enchanted by structural biology of membrane proteins! Congratulations! We will be sad to see you go in September, but we hope to see you every now and then in Prague or Grenoble (ESRF). All the best for your future.
Kudos!

Anežka joins the lab

02 06 2014

for a PhD

Anežka Tichá has joined us as a PhD student. Anežka (a beautiful traditional Czech name) has done her master thesis with Lenka Maletínská at the IOCB developing anti-obesity peptides (very successfully – watch that space). She has decided to switch to basic research for her PhD and took up the challenge to work on rhomboid-family proteins. Kudos!

Lucie has graduated!

01 06 2014

Lucie has passed her viva, defended her thesis and has graduated as a Master of Science from the Department of Biochemistry of Charles University! Congratulations, Lucka!

Celebrations followed…

Jakub wins a student grant!

28 03 2014

Jakub Began has won a student grant from the Charles University for his PhD project for the years 2014-2016. Well done! Congratulations!

Katka has joined the group

03 02 2014

Mgr. Kateřina Švehlová (Katka) has done her MSc and BSc work in Budweiss with Dr. David Dolezel and Dr. Lukas Trantirek. She now joins us as a research assistant. Welcome!

Postdoc fellowship for Nicholas

30 10 2013

Nicholas has been awarded the IOCB postdoctoral fellowship. Congratulations!

Postdoc fellowship for Sebastian

25 04 2013

Sebastian has won the IOCB postdoctoral fellowship. Congratulations!

PhD fellowship for Jan

26 02 2013

Jan Skerle has won a student grant from the Charles University for his PhD project for the years 2013-2015. Congratulations!

Nicholas has joined the group.

21 01 2013

Dr. Nicholas Johnson has earned his PhD in cell biology from the group of Prof. Steven High at the University of Manchester, UK. He has joined us as a postdoc. Welcome!

Sebastian has joined the group.

14 01 2013

Dr. Sebastian Zoll has done his PhD in protein crystallography in the group of Prof. Thilo Stehle at the University of Tuebingen, Germany. Welcome!

Lucie has joined the group.

01 12 2012

Lucie Peclinovska has done her BSc work in the lab of Leos Valasek at the Institute of Microbiology in Prague. Welcome!